For the quantitative determination of quinine, salicylic acid was used as internal standard at a concentration of 05 ng/µl, resulting in a detection limit (signal‐to‐noise ratio 3:1) of 03. Determine the quinine concentration in a diluted sample of tonic water finally, a urine sample taken within 24 hours of a subject's ingestion of tonic water will be tested for its quinine content. The tonic water using a least squares spreadsheet and report the appropriate uncertainties remember to calculate and report the concentration of quinine in the orginal tonic water in your discussion compare and contrast the excitation spectrum collected with. The instrument gives a coefficient of determination value if this value is less than 090, you must pour out your standard solutions and repipet them from the stock quinine solution.
Below is an essay on determination of quinine from anti essays, your source for research papers, essays, and term paper examples quinine-containing beverages may cause health problems updated bfr health assessment no 020/2008, 17 february 2005. By suitably diluting the tonic water provided (suggested dilution is 10cm3 to 100cm3) find its absorbance at 250nm and hence the concentration of the diluted tonic water calculate the concentration of quinine in the original tonic water in mg per litre. Abstract: an undergraduate laboratory experiment is designed for the quantitative determination of quinine in tonic water samples it is based upon direct fluorescence emission and first-derivative spectroscopic methods.
Quinine is a flavour component of tonic water and bitter lemon drink mixerson the soda gun behind many bars, tonic water is designated by the letter q representing quinine. Spectrofluorometric determination of quinine in tonic water experiment no 3 instrumental methods of food analysis - fdst 4080l/6080l by rb pegg this preview has intentionally blurred sections. Lab 9 fluorimetric determination of quinine in tonic water principles: much like in absorbance spectroscopy, a signal intensity is emitted when electron's fall down energy levels.
Quinine sulfate, stock standard: 100 mg/l weight out 302 mg quinine sulfate 2h 2 o and dissolve in 250 ml 01 n h 2 so 4 quinine standards, working: 5, 10 and 15 mg/l using 100 ml volumetric flask, dilute appropriate volumes of the quinine stock standard using 01 n h 2 so 4 as diluent. A 1 ml sample of tonic water (containing an unknown concentration of quinine sulphate (aq)) was diluted to the mark with the 005 mol/l h2so4 (aq) in a 100 ml volumetric flask a sample of this solution was placed in the spectrofluorometer and its spectrum, fluorescence intensity were recorded. A fluorometric analysis of quinine in tonic water introduction in this laboratory exercise, we will determine the amount of quinine in tonic water using a.
The tonic water sample was then examined at various dilutions (10x, 20x, 50x, and 100x) in order to find which dilution would be within the range of standards in order to have this unknown value fit within a calibration plot. For the quantitative determination of quinine, salicylic acid was used as internal standard at a concentration of 05 ng/µl, resulting in a detection limit (signal‐to‐noise ratio 3:1) of 03 ng, while the upper limit of linear range was 07 ng/µl. Quinine is an alkaloid derived from the bark of the cinchona tree it is used as an antimalarial drug, and is the active ingredient in extracts of the cinchona that have been used for that purpose since before 1633.
Chem 438 lab 4 fall 2003 lab 4 determination of quinine in tonic water by fluorescence introduction to the lab in a previous lab, you determined the concentration of quinine in. Determination of quinine in commercial soft drink by molecular fluorescence spectroscopy 1 introduction: quinine is an alkaloid ocuring naturally in the bark of trees or shrubs of the various species of two rubiaceous genera, cinchona and remijia, indigenous to the higher eastern slopes of the tropical andes in south america (1. Quinine contents of cloudy fruitdrinks can be rapidly determined by the fluorimetric method as described by strache (4) for tonic water a description of the procedure is given chiningehalte von trüben fruchtsaftgetränken können schnell and einfach bestimmt werden mittels einer fluorometrischen.
Quinine, best known for being added to drinks such as tonic water and bitter lemon, was originally used as an anti-malarial drug before being replaced by modern alternatives. experiment 2 title: determination of quinine in tonic water objective: to determine the concentration of quinine hydrochloride in sample (schweppers) given, by plotting a calibration curve of fluorescent intensity against concentration of quinine hydrochloride in ppm, after fluorescence intensity of a series of standard solution prepared and the sample solution by fluorescence spectrometer. Quinine is a bitter alkaloid that is used as a flavoring agent in tonic water studies suggest that quinine can inhibit cytochrome p450 2d6 (cyp2d6) which could have implications for the metabolism of co-ingested drugs. Chem 438 lab 2 fall 2003 laboratory 2 determination of quinine in tonic water by uv-vis absorbance spectroscopy introduction to the lab the purpose of this lab is to use the uv-vis spectrophotometer to.